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The Journal of Heredity 1942:33(5):173-184
© 1942 The American Genetic Association 33:173-184

research-article

METHODS FOR ISOLATING, COLLECTING AND ORIENTING SALIVARY GLAND CHROMOSOMES FOR DIFFRACTION ANALYSIS

JOHN B. BUCK, and A. M. MELLAND

University of Rochester
Cambridge University*

Abstract

Two methods for isolating salivary gland nuclei from unfixed glands and three methods for isolating nuclei and single chromosomes from fixed glands are described. Techniques for collecting these nuclei and chromosomes and mounting them in various ways for X-ray and electron diffraction photography are described. The photographs taken to date have not justified any definite conclusions regarding the structure of salivary gland chromosomes, but the technique has not been adequately tested. It is pointed out that the methods may be of value in physiological and biochemical investigations, and in electron microscopy.


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