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The Journal of Heredity 1981:72(2):81-86
© 1981 The American Genetic Association 72:81-86

research-article

Chinese hamster ovary chromosomes and antigens in tobacco/hamster heterokaryons

Iris A. Mastrangelo, and J. Mitra

The authors are, respectively, guest biologist, Biology Department, Brookhaven National Laboratory, Upton, NY 11973; and Biology Department, New York University, Washington Square, New York, NY 10003. They thank H. H. Smith, Biology Department, Brookhaven National Laboratory for his helpful discussions and critical review of the manuscript. This research was supported by NSF grant PCM 77-14909. Please address reprint requests to Dr. Mastrangelo.

Abstract

Tobacco GGLL/hamster CHO hamster heterokaryon frequency following cell fusion has been raised to 4–10 percent with a modified PEG procedure. In heterokaryons, GGLL nuclei become activated, adhere to and appear to fuse with CHO nuclei. Metaphase CHO chromosomes can be identified 24 hours after fusion and immunofluorescent CHO cell surface antigens diffuse into the plant cell membrane. Diffusion coefficients, D, are estimated to be between 0.6 x 10–11and 1.6 x 10–11 cm2sec–1


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