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The Journal of Heredity 1982:73(1):74-76
© 1982 The American Genetic Association 73:74-76

other

A rapid method for identification of constitutive heterochromatin of secondary constriction regions of human chromosomes 1, 9, and 16

R. S. Verma, S. Ved Brat, and H. Dosik

Divisions of Cytogenetics and Hematology of the Jewish Hospital and Medical Center of Brooklyn 555 Prospect Place Brooklyn, NY 11238; and the Department of Medicine, State University of New York Downstate Medical Center Brooklyn, NY.

Abstract

The secondary constriction regions (h) of chromosomes 1, 9, and 16 stained deep red by RBA (R-bands by BrdU using Glemsa)—a useful tool for demonstrating the constitutive heterochromatic heteromorphisms in these regions. The unusual staining pattern of bands p12 to q11 of chromosome 3, which is late replicating, also is reported. The extended deep red region does not correspond to C-banding on chromosome 3. This technique does not require sequential banding by QFQ and CBG for detecting the h-region hetermorphisms.


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