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The Journal of Heredity 1982:73(2):151-152
© 1982 The American Genetic Association 73:151-152

other

Uptake of isolated lily chromosomes by tobacco protoplasts

R. J. Griesbach, R. L. Malmberg, and P. S. Carlson

Nursery Crops. USDA-BARC West Beltsville, MD 20705: Laboratory, Cold Spring Harbor NY, 11724; and professor. Department of Crop and Soil Science Michigan State University

Abstract

Using fluorescence microscopy a vlsu-system was developed which allowed the monitoring of the uptake of isolated chromosomes by plant protoplast under various conditions. The optimal conditions were a 20-minute exposure to a 35 percent polyethylen glycol solution. Under these conditions about 1 percent of the recipient protoplasts contained a foreign chromosome.


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