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The Journal of Heredity 1982:73(3):192-196
© 1982 The American Genetic Association 73:192-196

research-article

Isolation of nullihaploids from diverse genotypes of Nicotiana tabacum

Gloria A. Moore, and Glenn B. Collins

Department of Agronomy, University of Kentucky Lexington, KY 40546

Abstract

In Nicotiana tabacum, nullihaploid plants can be produced through the in vitro culture of anthers from monosomic plants. The chromosome numbers of the isolated nullihaploids can then be doubled to produce 46 chromosome nullisomics. This study was designed to examine factors involved in the production of nullihaplolds from anther cultures and to increase the efficiency of nullihaploid production. The effect of culturing monosomic anthers from several genetic backgrounds was assessed. No genotype was found to be superior to the ‘Purpurea’ or ‘Red Russian’ genotype in nullihaplold production. Experiments also were carried out to evaluate the effect of competition between 23- and 24-chromosome microspores on nullihaploid production. Anther cultures of double monosomics and other genotypes that produced fewer 24-chromosome microspores than were produced from monosomic anthers were evaluated. Such genotypes were much more productive of nullihaploids than were monosomic cultures, demonstrating that competition between microspores is an important factor in the production of some nullihaplold types. The number of nullihaploids obtained from anther cultures of N. tabacum could be greatly increased by the use of genotypes that produce fewer 24-chromosome microspores.


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