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The Journal of Heredity 1983:74(4):251-259
© 1983 The American Genetic Association 74:251-259


research-article

Inheritance of acid phosphatase and NAD-malate dehydrogenase isozymes in Tetrahymena pigmentosa

Rosa Maria Preparata, David L. Nanney, and Ellen M. Simon

the Department of Genetics and Development, University of Illinos Urbana, IL 61801

Abstract

The cytosol NAD malate dehydrogenase (MDH) in Tetrahymena pigmentosa is a dimeric enzyme associated with a locus mdh for which three allelic forms are described. The products of the alleles combine freely in heterodimers. Similarly, the most anodal acid phosphatase (ACP) also is a dimeric enzyme associated with an unlinked locus acp, for which three alleles are described. Heterozygotes at either locus manifest allelic assortment during vegetative growth; young heterozygotes usually manifest both homodimers and the appropriate heterodimers, but sublines produced after serial isolations often show only a single homodimer. Such "assorted" sublines nevertheless transmit to their progeny both allelic forms; the micronuclei do not undergo vegetative assortment. Thus, allelic assortment that occurs for all loci in T. thormophila occurs at least for these two loci in T. pigmontosa, even though it may not be characteristic of all genetic loci in the species.


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