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The Journal of Heredity 1984:75(4):269-272
© 1984 The American Genetic Association 75:269-272

research-article

R-banding of horse chromosomes

The fluorescence-photolysis-Giemsa technique after bromodeoxyuridine incorporation

A. Romagnano, and C. -L. Richer

Département d'Anatomic, Faculté de Médecine, and with the Centre de Recherche en Reproduction Animale, Faculté de Médecine Vétérinaire, Université de Montréal C.P. 6128. Succursale A, Montréal, Québec, Canada H3C 3J7

Abstract

To date, equine chromosomes have been evaluated primarily after C-banding (CBG) and G-banding (GTG). Two types of R-banding also have been described: those produced by heat denaturation (RHG), and those by fluorescent acridine orange staining after bromodeoxyuridine (BrdU) incorporation (RBA). However, these have failed to give sufficient detail (GTG, CBG), are difficult to reproduce (RHG), or require fluorescent microscopy (RBA). We have successfully applied to equine chromosomes the simple R-banding method using BrdU, the fluorescent 33258 Hoechst stain followed by black light exposure and Glemsa staining (RB-FPG). Compared to other R-banding techniques the RB-FPG method produces a very precise banding pattern. It enables definite identification and easy pairing. We consider this a most reliable and useful technique for studying horse chromosomes.


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