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The Journal of Heredity 1985:76(1):17-20
© 1985 The American Genetic Association 76:17-20

research-article

An apparent meiotic mutation in a mesophyll cell protoclone of the ‘Russet Burbank’ potato

B. S. Gill, L. N. W. Kam-Morgan, and J. F. Shepard

The authors are affiliated with the Department of Plant Pathology, Kansas State University, Manhattan, KS 66506; present address of Dr. Shepard: Allelix, Inc., 6850 Goreway Drive, Mississauga, Ontario, L4V 1PI, Canada. Contribution no. 84-486-J, Department of Plant Pathology, Kansas Agricultural Experiment Station, Kansas State University, Manhattan. Research supported by NSF grant PCM 8003842.

Abstract

Plants regenerated from mesophyll cells (protoclones) were analyzed for a single gene mutation. Russet Burbank potato (Solanum tuberosum L.) is homozygous recessive for a gene(s) that causes spindle abnormalities leading to first division restitution (FDR), second division restitution (SDR), and double restitution (DR). As a result, no hapiold pollen is produced in Russet Burbank. Mesophyll cell protoplasts from Russet Burbank were regenerated into plants and screened for mutations at this locus. Among 55 protoclones, three plants showed 20–30 percent, one plant showed 69 percent and mutant M248 showed 83 percent reduction in spindle abnormalities. The 20–69 percent reduction in the four protociones resulted from a reduced frequency of one or more types of spindle abnormalities but only mutant M248 showed a consistent and drastic reduction in all spindle abnormalities. Thus, M248 produced 83 percent haploid pollen while Russet Burbank produced none. It was apparent that M248 may contain a single, dominant meiotic mutation. The results indicate a high mutation rate in cell cultures of Russet Burbank potatoes.


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