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The Journal of Heredity 1985:76(5):375-376
© 1985 The American Genetic Association 76:375-376

other

Cell synchronization and dynamic G-banding of equine chromosomes by bromodeoxyurldine

C. L. Richer, and A. Romagnano

The authors are affiliated with the Département d'Anatomie, Faculté de Médecine, Université de Montréal, CP. 6128, Succ. A, Montréal, Québec, Canada H3C 3J7; and with the Centre de Recherche en Reproduction Animale, Faculté de Médecine Vétérinaire, Université de Montréal, Montréal, Québec, Canada. Part of this work was presented at the 6th European Colloquium on Cytogenetics of Domestic Animals, Zurich, July 1984. The research was supported by the "Fonds F.C.A.C. pour l'aide et le soutien à la recherche", Québec. The authors wish to thank Elise Ménard-Landry, Brigitte L'Hermite-Beugnot, and Louise Laquerre for helpful assistance. Please address reprint requests to Dr. Richer.

Abstract

Both dynamic G-banding and cell synchronization produced by bromodeoxyuridine (BrdU), were applied to equine chromosomes. BrdU incorporated during the first half of the S-phase is taken up into the R-bands that are early replicating. These bands, which have incorporated BrdU, cannot contract as usual and remain elongated; only the other regions of the chromosome, i.e., the G-bands, contract normally and are sharply defined. BrdU also can be used for cell synchronization. The addition of BrdU in a high concentration, 15 hours before harvest, and its removal 11 hours later, has two effects: initially the BrdU is incorporated during the first part of the S-phase and then it blocks the cells at mid-S-phase. Within the cell cycle, mid-S-phase appears to be the most vuinerable time to various blocking agents. To differentiate the regions of BrdU incorporation from those that have not been substituted, the fluorescence-photolysis-Glemsa (FPG) technique was applied as modified for horse chromosomes. This dynamic technique, which produces many prometaphase and prophase chromosomes showing very sharp G-bands, is certain to enhance the accuracy of cytogenetic analysis and aid in the standardization of equine chromosomes.


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