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The Journal of Heredity 1985:76(6):406-414
© 1985 The American Genetic Association 76:406-414


research-article

A chromosome translocation breakpoint map of the Gossypium hirsutum genome

Margaret Y. Menzel, Kenneth L. Richmond, and Brian J. Dougherty

The authors are, respectively, professor, biologist II, and doctoral student, Department of Biological Science, Florida State University, Tallahassee, FL 32306. This work was supported by grants no. 5901-0410-8-0116-0, 59-2112-1-1-6360, and 84-CRCR-1-1383 from the Competitive Research Grants Office, U. S. Department of Agriculture. The technical assistance of Karen Graffius, Leslie B. Campbell, Carol Contolini, and many undergraduate research assistants is gratefully acknowledged. The authors also thank Dr. Clare A. Hasenkampf for assistance in early stages and for helpful discussions throughout the study. Stephen Bradley prepared the diagrams in Figure 1.

Abstract

A recombination map of the cotton genome was constructed based on chiasma frequencies in chromosome regions defined by the breakpoints in 58 reciprocal chromosome transiocations. Total lengths of 50 arms were estimated in recombination units, and the arm locations and distances from the centromeres of 115 breakpoints were mapped. The breakpoints appear to be distributed between the Ah and Dh genomes in proportion to the relative amounts of DNA in each genome. However, the proximal clustering of breakpoints on the map suggests that there is a tendency for one chiasma per arm to be localized distally. Additional chiasmata may be more randomly distributed.


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