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The Journal of Heredity 1986:77(4):253-255
© 1986 The American Genetic Association 77:253-255

research-article

Molecular identification of the D-genome chromosomes of wheat

A. Lane Rayburn, and B. S. Gill

The authors are, respectively, research associate and associate professor, Department of Plant Pathology, Kansas State University, Manhattan, KS 66506. Contribution no. 86-224-J, Department of Plant Pathology, Kansas Agricultural Experiment Station, Kansas State University, Manhattan.

Abstract

A highly repeated DNA sequence isolated from Aegllops squarrosa was used to Identify specific chromosomes in hexaploid wheat. The DNA sequence found In clone pAS1 appears D-genome specific and, therefore, allows for the unambiguous identification of the D-genome chromosomes. In situ hybridization using blotin labeled DNA probes was found to be a rapid and reliable technique to identify sites of hybridization on D-genome chromosomes. Multiple sites of hybridization of the sequence allowed the Identification of all seven pairs of the D-genome chromosomes.


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