The Journal of Heredity 1986:77(6):435-440
© 1986 The American Genetic Association 77:435-440
research-article |
Chicken developmental antigens in 15I5-B-congenic lines
The authors are, respectively, graduate student, graduate student, and associate professor of immunogenetics, Department of Poultry and Avian Sciences, Cornell University, Ithaca, NY 14853; and immunologist, U.S. Department of Agriculture, Agricultural Research Service, Regional Poultry Research Laboratory, East Lansing, MI 48823. The authors thank F. Sanders and M. Blab for their excellent technical support, D. Colf for assistance in the preparation of the manuscript, and Dr. T. Fredericksen for his critical reading of the manuscript. Drs. B. Calnek and K. Schat are gratefully acknowledged for providing Marek's disease vaccine. Supported in part by Cornell Biotechnology grant 157302, grant NY(C) 157424 from the USDA, and by USDA contract for work at the Regional Poultry Research Laboratory. Mention of a trade name, proprietary product, or specific equipment does not constitute a guarantee or warranty by the U.S. Department of Agriculture and does not imply its approval to the exclusion of other products that may be suitable.
Abstract
Six partially developed 15I5-B-congenic lines of chickens were used to assess the genetic influence on the developmental expression of selected epitopes of two avian developmental antigen systems: chicken fetal antigen (CFA) and chicken adult antigen (CAA). Both CFA and CAA are serologically and molecularly complex hematopoletic antigen systems, yet little is known about genetic Influences on their expression. Using polyclonal rabbit anti-CFA, only slight variations in overall CFA expression on peripheral erythrocytes were observed during neonatal development; no consistent trend was evident. In contrast, analysis with monoclonal antibody 10C6 revealed that the incidence of CFA determinant 8 (CFA8) on erythrocytes of the early neonate was significantly reduced in line 15I5 compared with lines.6–2.7–2 and 151–5; line. C-12 also exhibited a reduced CFA8 Incidence at hatching. Likewise, the CAA epitope detected by monoclonal antibody 3F12 was found to appear at a slower rate on erythrocytes from lines 15I5 and C-12 than on those of other lines. Similar results were obtained using the anti-CAA monoclonal 4C2 where reduced expression was found in lines 15I5, C-12, and P-13. Results of complement-mediated cytolysls using the positive control 9F9 monoclonal antibody suggested that observed genetic differences were not due to inherent differences in erythroid cytolytic sensitivity. Neither could the results be explained by the incidence of circulating reticulocytes vs. mature erythrocytes within the lines. Rather, the results suggest that different genetic lines of chickens vary in the developmental kinetics of definitive erythrocyte subpopulations bearing specific phenotypes defined by monoclonal antibodies. These findings are discussed in light of previous observations using these B-congenic lines.