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The Journal of Heredity 1987:78(3):178-182
© 1987 The American Genetic Association 78:178-182

research-article

Hypotonic bursting method for spreading synaptonemal complexes of Zea mays

Stephen Stack, and Lorinda Anderson

Department of Botany and the Cell and Molecular Biology Program, Colorado State University Fort Collins, CO 80523

Abstract

An adaptation of the hypotonic bursting technique for spreading synaptonemal complexes (SCs) is described for primary microsporocytes of Zea mays. SCs are spread in two dimensions and stained with silver for observations in the light or electron microscope. Substages of pachytene can be determined accurately using changes in the structure of the nucleolus. This technique allows detailed analyses of the synaptic pattern in complete sets of SCs, including synaptic irregularities such as inversion loops.


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