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The Journal of Heredity 1987:78(3):187-190
© 1987 The American Genetic Association 78:187-190

research-article

Genetic polymorphism, mapping, and characterization of isocitrate dehydrogenase in Anopheles quadrimaculatus

Sang Seock Kim, S. K. Narang, and J. A. Seawright

Korea Ginseng and Tobacco Research Institute P.O. Box 59, Suweon, Korea
Insects Affecting Man and Animals Research Laboratory USDA, ARS, Gainesville, Florida, 32604

Abstract

The isocitrate dehydrogenase-2 (idh-2) locus of Anopheles quadrimaculatus was analyzed genetically and the enzyme was characterized physiochemically. Three–point testcrosses involving chromosome 3 markers showed that in female hybrids the gene sequence and the map distances are: nonstripe (st)—6.8—idh-2—43.5—short antenna (Sa). Reduced recombination frequencies were observed in male hybrids (st—3.4—idh-2—25.5—Sa). idh-2 activity gradually increases during development and reaches a peak intensity in adults. Maximum enzyme activity of idh-2 was obtained at pH 7.5. One-minute heat treatment at 50°C caused about 50 percent reduction of IDH-2. Ethylene diamine tetraacetic acid (EDTA 5mM) and p-chloromercuribenzoate (pCMB 10–5M) caused complete loss of activity of IDH-2, but pretreatment of the enzyme in situ with mercaptoethanol protected the activity of allozymes from inhibition by pCMB treatment.


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