Skip Navigation

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrowRequest Permissions
Google Scholar
Right arrow Articles by Newell, C. A.
Right arrow Articles by Edge, M. E.
Right arrow Search for Related Content
PubMed
Right arrow Articles by Newell, C. A.
Right arrow Articles by Edge, M. E.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

The Journal of Heredity 1987:78(5):301-306
© 1987 The American Genetic Association 78:301-306

research-article

Interspecific hybrids between the soybean and wild perennial relatives

C. A. Newell, X. Delannay, and M. E. Edge

The authors are, respectively, research specialist, research group leader, and former research biologist I (currently research assistant, Department of Agronomy and Range Science, University of California, Davis, CA 95616), Monsanto Company, 700 Chesterfield Village Parkway, St. Louis, MO 63198. A part of this research was conducted by C. A. Newell at Advanced Genetic Sciences, Inc., 6701 San Pablo Avenue, Oakland, CA 94608. She gratefully acknowledges Advanced Genetic Sciences for its support in this project.

Abstract

Successful crosses were obtained in 390 different genotype combinations between the soybean, Glycine max (L.) Merr., and wild perennial Glycine species. A total of 1534 Immature embryos were excised prior to abortion and cultured, and 1.4 percent of these germinated to produce shoots representing 18 genotype combinations. Sixteen F1 Interspecific hybrids encompassing 12 different combinations were subsequently grown to maturity: 7 G. max (2n = 40) x G. tomentella Hayata (2n = 78), 3 G. tomentella x G. max, and 2 G. canescens F. J. Herm. (2n = 40) x G. max. The F1 hybrids differed morphologically depending on the genotype combination, and showed variable degrees of anther development. Hybrids with wild Glycine as the female parent gave the most normal anther development. Expected chromosome numbers of 2n = 59 and 2n = 40 were confirmed for G. tomentella and G. canescens hybrids, respectively. The F1 hybrids exhibited low chromosome pairing and perturbed melosis in pollen mother cells and were sterile. Eight hybrid combinations were doubled with colchicine to give 2n = 118 and 2n = 80 chromosomes for G. tomentella and G. canescens hybrids, respectively. Doubled plants exhibited high levels of chromosome pairing as expected, but remained highly sterile because of abnormal microspore formation. Fertility was partially restored in one G. max x G. tomentella (2n = 118), which set mostly one-seeded, cleistogamous pods at a low rate. Chromosome numbers of selfed progeny were similar to the doubled parent. Variation in viability and fertility shown by different hybrid combinations within one interspecific cross indicate that genotype interactions play a large part in determining the ease with which hybrids can be made between wild perennials and the soybean, and will most likely influence which Glycine collections represent potential candidates for gene exchange with the soybean.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?




Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.