The Journal of Heredity 1987:78(5):323-326
© 1987 The American Genetic Association 78:323-326
research-article |
Genetic and linkage analysis of an aconitate hydratase variant in the soybean
The authors are graduate research assistant and associate professor, Crop Science Department, University of Guelph, Guelph, Ontario NIG 2WI, and soybean breeder, Agriculture Canada Research Station, Harrow, Ontario N0R IG0. They greatly appreciate the technical assistance of Matian Thorpe and Cathy Mann. Brad Hedges provided data from his analysis of the G. max and G. soja accessions. Agriculture Canada and the Soybean Growers' Marketing Boart provided funds for this research. Thanks are extended to the Soybean Genetics committee for reviewing this article.
Abstract
Five bands are seen when electrophoretic starch gels of emerged soybean (Glycine max (L.) Merr.) cotyledons taken from pure lines are stained for the enzyme aconitate hydratase (ACO, EC 4.2.1.3 [EC] ). Only four bands were observed, the central band being absent, with samples of root or leaf tissue or germinating seed. Two mobility variants for a single band, band 4 (the second most anodal) are the result of codominant alleles at a single locus. This locus has been designated Aco4 as the band affected is the fourth band from the origin. The two alleies have been assigned the genetic symbols Aco4-a and Aco4-b for the slow (Rf. 0.32) and fast (Rf. 0.37) migrating bands, respectively. The Aco4 locus segregated independently of three electrophoretic markers (Dia 1, ldh1, and Pgm 1), four morphological markers (Dt 1, Ln, T, and W1), and two disease resistance loci (Rps 1 and Rps6). Based on accumulated zymogram results for a number of G. max and G. soja Sleb. & Zucc. strains, the Aco4-b allele occurs more frequently than the Aco4-a allele.