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The Journal of Heredity 1988:79(4):239-248
© 1988 The American Genetic Association 79:239-248

research-article

Characterization of Different B-F (MHC Class I) Molecules in the Chicken

K. Kline, W. E. Briles, L. Bacon, and B. G. Sanders

Division of Nutrition, University of Texas Austin
Department of Zoology, University of Texas Austin
Department of Biological Sciences, Northern Illinois University DeKalb
Regional Poultry Research Laboratory, Agricultural Research Service, U.S. Department of Agriculture East Lansing, Michigan

Address reprint requests to Dr. K. Kline, Assistant Professor, Division of Nutrition, Department of Home Economics, University of Texas, Austin, TX 78712.

Abstract

B-F alloantisera recognized distinct 45-Kd molecules on peripheral red blood cells (RBC) from embryonic chickens and heterogenous molecules of approximately 40 to 44 Kd on peripheral RBC from adult chickens, provisionally referred to as type 1 and type 2, respectively. Type 2 molecules migrated to the basic end of Isoelectric focusing gels, exhibited multiple isomorphic variants, and were associated with a smaller polypeptide of approximately 11 to 12 Kd assumed to be ß-2-microglobulin. Type 1 molecules migrated to the acidic end of isoelectric focusing gels, exhibited limited heterogeneity, and were not associated with a smaller polypeptide. Type 1 and type 2 molecules were also shown to be distinct by peptide mapping and serological analyses. In addition, two distinct molecular-weight forms of the type 2 molecules were distinguished, provisionally referred to as 2A (45 Kd) and 2B (42 Kd). In vivo–derived avian erythroblastosis virus (AEV)-transformed erythroleukemia cells expressed type 2A molecules. In vitro–derived AEV–transformed erythroleukemia cells expressed very low levels of B-F molecules; however, they expressed type 2B molecules when induced to differentiate. Normal bursa-derived lymphoid cells expressed type 2A molecules, whereas normal thymus-derived lymphoid cells expressed type 2B molecules. Cloned reticuloendotheliosis virus (REV)–transformed immature lymphoid cells expressed either type 2A or type 2B molecules.


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