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The Journal of Heredity 1988:79(6):469-472
© 1988 The American Genetic Association 79:469-472

research-article

An Evaluation of Maternal Nullihaploidy for Nicotiana tabacum L. Nullisomic Production. II. A Pollen Irradiation and Ovule Culture Approach

M. G. Kramer, and S. M. Reed

Department of Crop Science, North Carolina State University Raleigh

Address reprint requests to Dr. Reed, Department of Crop Science, North Carolina State University, Raleigh, NC 27695-7620.

Abstract

The combined use of an irradiated pollen-induced maternal haploidy procedure and ovule culture for the production of nullihaploids of Nicotiana tabacum L. was tested Monosomics F, H, L, M, P, and R of the N. Tabacum genotype ‘Purpurea’ were pollinated with irradiated (50 krad: 60Co) N. glutinosa L. pollen. Fourteen to 16 days after pollination, the ovules were cultured on a chemically defined artificial nutrient medium Of the 58 plants obtained from the ovule cultures, 3 were haploids and 53 were aneuploid interspecific hybrids. The remaining two plants, which came from a single monosomic H cross, were nullihaploids (n = 23). The chromosome number of these plants was doubled through leaf midvein culture to produce nullisomics (2n = 46) Meiotic analysis of the nullisomics revealed the presence of a few univalents. How ever, the degree to which chromosome pairing was disrupted by the absence of chromosome H could not be determined. The presumed nullisomic H plants were male- and female-sterile. Therefore, the usefulness of this particular nullisomic for genetic analysis is extremely limited.


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