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The Journal of Heredity 1989:80(4):291-297
© 1989 The American Genetic Association 80:291-297

research-article

Genetic Control of Isozyme Variation in Needle Tissues of Cryptomeria japonica

Y. Tsumura, K. Uchida, and K. Ohba

Institute of Agncuiture and Forestry, University of Tsukuba Japan

Address reprint requests to Dr. Tsumura, Institute of Agriculture and Forestry, University of Tsukuba. Tennodai, Tsukuba, Ibaraki 305, Japan

Abstract

Using polyacrylamide vertical slab gel electrophoresis, we developed methods for detecting allozyme variants of Cryptomeria japonica from needle tissues. We ex amined 18 enzyme systems to detect marker genes; clear zymograms were obtained for 13 enzyme systems. We observed electrophoresis banding variants for nine of these 13 enzyme systems. These variants apparently are controlled by at least 29 genes in 14 loci.


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