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The Journal of Heredity 1989:80(6):442-446
© 1989 The American Genetic Association 80:442-446

research-article

Mapping of PRM 1 to Human Chromosome 16 and Tight Linkage of Prm-1 and Prm-2 on Mouse Chromosome 16

R. H. Reeves1, J. D. Gearhart1, N. B. Hecht2, P. Yelick2, P. Johnson2, and S. J. O'Brien3

1From the Developmental Genetics Laboratory, The Johns Hopkins University School of Medicine Baltimore, Maryland
2Department of Biology, Tufts University Medford, Massachusetts
3Laboratory of Viral Carcinogenesis National Cancer Institute Frederick, Maryland

Address reprint requests to Dr. Reeves, Department of Physiology, P202, The Johns Hopkins University School of Medicine, 725 North Wolfoe St. Baltimore, Md 21205.

Abstract

The protamines are small, arginine-rich nuclear proteins that replace histones and transition proteins late in the haploid phase of spermatogenesis in mammals. The two mouse genes encoding protamines–Prm—1 and Prm—2–have been molecularly cloned and mapped to mouse chromosome 16 (MMU 16). A cDNA clone of mouse Prm—1 that hybridized to the corresponding human gene was used to analyze a panel of somatic cell hybrids made between human lymphoblasts and the E36 hamster cell line. The human gene, which we have designated PRM1, was syntenic with human chromosome 16 (HSA 16) and discordant with all other human chromosomes. Linkage analysis in the mouse was accomplished using the backcross (Czech II × BALB/c Pt) × Czech II to map Prm-1 and Prm-2 to a position near the 5’ terminus of MMU 16. No recombination between Prm-1 and Prm-2 was observed among 89 progeny of the Czech II × BALB/c cross or among 94 progeny of the backcross (CBA/J × BALB/cJ) × BALB/cJ, demonstrating that the two loci are separated by less than 1.6 cM on MMU 16. This tight linkage may be of functional significance, as Prm-1 and Prm-2 are among a limited number of genes known to be expressed postmeiot-ically in male haploid germ cells.


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