Skip Navigation

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrowRequest Permissions
Google Scholar
Right arrow Articles by Stelly, D. M.
Right arrow Search for Related Content
PubMed
Right arrow Articles by Stelly, D. M.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

The Journal of Heredity 1990:81(3):193-197
© 1990 The American Genetic Association 81:193-197

research-article

Localization of the Le2 Locus of Cotton (Gossypium hirsutum L.)

D. M. Stelly

Texas Agricultural Experiment Station. The reserarch was supported by USDACRGO Grant No. 86-CRCR-1-2129

Abstract

Hybrid lethality systems are widely distributed among angiosperms and are of biological, evolutionary, and genetic interest, as well as being of potential importance for genetic manipulations. The Le2dav allele occurs naturally in the two Gossypium D3-genome species, G. davidsonii and G. klotzschianum, isolating them from other species of the cotton genus that carry A or D genomes. Le2dmv was previously transferred to G. hirsutum and found to be allelic to the Le2 locus of chromosome 26. Le2dmv was shown to interact differentially with its allele Le2 and its paralogous allele in chromosome 12, Le,. In this study, monotelodisomic tests were made to locate the Le2 locus to an arm of chromosome 26. A potentially hemizygous Le1Le1Le2(Le2) plant monotelodisomic for the short arm of chromosome 26 (Te26sh) was crossed with le1le1Le2davLe2dev Euploid F1S1 progenies from euploid (Le, Lef, Le2,e2) and Te26sh (Le, le, (LeJ, le2) F, plants were testcrossed with le, leJ Le, lai ‘Le2da1’, and the resulting FtSjTC families were scored to determine genotypes of respective F1S1 parents. Segregation among the F^TC families indicated that the Le2 locus is located in the long arm of chromosome 26, or within 5 cM of the centromere in the short arm. The data also revealed that the LerLe2dav interaction caused necrosis more quickly than did the Le2-Le2dav interaction. Individual F^TC plant segregations fit poorly with Mendelian expectations. Estimates of differential emergence for F^TC seed genotypes were used to amend raw data in an unbiased manner, resulting in better fits to expected segregation ratios and increased homogeneity among families. The results lend credence to previous inferences that differential viability can cause non-Mendelian ratios among individual plants segregating for alleles of this hybrid lethality system, and also indicate that it should be possible to use Te26sh stocks to develop isolines differing at the Le2 locus. Such stocks would facilitate comparisons of Le2 alleles from different sources and thus the analysis of polymorphisms of the Le2 locus, which might further our understanding of how hybrid lethality and sublethality systems evolve and contribute to speciation.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?




Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.