The Journal of Heredity 1990:81(3):198-200
© 1990 The American Genetic Association 81:198-200
research-article |
Twin Seedlings and Haploids in Blueberry (Vaccinium spp.)
From the Fruit Crops Department, University of Florida Gainesville
Address reprint requests to P. M. Lyrene, Fruit Crops Department, University of Florida, 1137 Fifield Hall, Gainesville, Fl 32611.
Abstract
One method of obtaining haploids in higher plants is to screen germinated seed for the presence of twin seedlings. Multiple seedlings were observed from seeds germinated in the greenhouse from open-pollinated Vaccinium ashei, 6x; V. corymbo-sum, 4x; V. elliottii, 2x; and V. darrowi, 2xat a frequency of 0.074%, 0.066%, 0.037%, and 0.033%, respectively. About 40% of the twin pairs consisted of one strong and one weak individual. Weak individuals did not survive under greenhouse conditions. Screening about 15, 000 V. ashei seedlings germinated in vitro produced 13 twin pairs, including 3 triploid/hexaploid pairs as determined by chromosome counts. Based on the inheritance of an anthocyanin-deficient marker gene, the origin of haploids in twins obtained from V. elliottii appeared to be maternal.