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The Journal of Heredity 1990:81(6):438-442
© 1990 The American Genetic Association 81:438-442

research-article

Protoplast-Fusion-Derived CMS Potato Cybrids: Potential Seed-Parents for Hybrid, True-Potato-Seeds

A. Perl, D. Aviv, and E. Galun

Department of Plant Genetics, Weizmann institute of Science Rehovot 76100, Israel.

Address correspondence to Professor Esra Galun, Department of Palnt Genetics, the Weizman Institute of Science, Rehovot 76100, Israel.

Abstract

F1 hybrid true-seeds of potato have potential advantages, over tuber-seeds, for planting potato fields under certain conditions, especially in developing countries. To eliminate laborious hand emasculation for the production of F1 seeds, we investigated the possibility of transferring cytoplasmic male sterility from an alloplasmic male sterile donor into two male fertile potato cultivars that were previously used as seed-parents in F1 hybrid seed-production. Protoplasts of cultivars ‘Atzimba’ and ‘Atlantic’ were thus used as recipients, exposed to iodoacetate or rhodamine-6G and fused with {gamma}-irradiated protoplasts of an alloplasmic Solanum tuberosum (stoloniterum) line (Y245.7). A total of 24 putative cybrid calli were obtained, from which 120 plants were regenerated. All the latter were similar to the recipient‘s morphology and flower pigmentation. Forty-one plants had no fertile pollen. In two of the latter cybrids, pollen development was arrested in the tetrad stage and one plant had petiolated anthers. The mitochondrial DNA restriction profile of the putative cybrid plants differed from those of either the donor of the recipient fusion-partner. The donor-recipient protoplast-fusion can thus serve to convert fertile potato cultivars into useful seed-parents for F1 hybrid-seed production.


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