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The Journal of Heredity 1991:82(6):509-512
© 1991 The American Genetic Association 82:509-512

research-article

Identification of D-Genome chromosomes of chinese spring of sourthern Blots using Gnome-specific repetative DNA

L.E. Talbert, and T.H. Clack

From the Department of Plant and Soil Science, Montana State University Bozeman, MT 59717

Address reprint requests to L. E. Talbert at the address above.

Abstract

Two D-genome-specific repetitive DNA probes were hybridized to restricted DNA from D-genome disomic substitution lines. These lines contained a single D-genome chromosome substituted for its A or B genome homoeologue in Langdon durum wheat. Both probes hybridized to DNA on every D-genome chromosome. One of the probes (pAS1) revealed a unique hybridization pattern for each chromosome using a single restriction enzyme, and thus it may be useful for the construction and monitoring of D-genome aneuploid wheat stocks.


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