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The Journal of Heredity 1992:83(1):36-42
© 1992 The American Genetic Association 83:36-42

research-article

Early and Late Replicative Chromosomal Banding Patterns of Gallus domesticus

F. A. Ponce de Leon, Y. Li, and Z. Weng

From the Department of Veterinary and Animal Sciences, 315 Paige Laboratory, University of Massachusetts Amherst, MA 01003

Address reprint requests to Dr. F. Abel Ponce de Leon at the address above.

Abstract

Early and late replicating chromosomal banding patterns of Gallus domesticus were investigated by cell synchronization and incorporation of 5'-bromodeoxyuridine during early and late DNA synthesis. The early replicating chromosomal banding patterns observed, as revealed by either acridine orange or Hoechst 33258/propidium iodide staining, were similar to the structural G-banding patterns obtained by trypsin di-gestion and Giemsa staining. Late replicating chromosomal banding showed extensive reverse band complementarity to the G-banding pattern. Cell synchronization increased the number of prometaphase and metaphase plates available for analysis. G-banding obtained by Hoechst 33258/propidium iodide staining was investigated due to the fact that it is compatible with chromosomal in situ hybridization procedures that use nonisotopically-labeled DNA probes. Standard replicative G-banded and R-banded idiograms, as obtained after cell synchronization, are proposed.


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