Skip Navigation

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrowRequest Permissions
Google Scholar
Right arrow Articles by Guo, M.
Right arrow Articles by Mok, D. W. S.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Guo, M.
Right arrow Articles by Mok, D. W. S.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

The Journal of Heredity 1994:85(3):174-178
© 1994 The American Genetic Association 85:174-178

research-article

RFLP Analysis of Preferential Transmission in Interspecific Hybrids of Phaseolus vulgaris and P. coccineus

M. Guo, M. C. Mok, and D. W. S. Mok

Department of Horticulture and Center for Gene Research and Biotechnology, Oregon State University Corvallis, OR 97331

Abstract

The inheritance of RFLP markers in interspecific hybrids of Phaseolus vulgaris and P. coccineus was analyzed. Of 280 cDNA probes used, 70%-85% revealed polymorphism between species while intraspecific RFLP ranged from 8% to 37%. Segregation of 63 clearly scorable markers was examined in 177 P. vulgaris × P. coccineusF2's maintained as callus. Preferential transmission of the P. vulgaris alleles was observed for 24 of the 28 loci exhibiting non-Mendelian ratios. Although the segregation ratios at 17 loci fit gametic selection, also other factors such as nuclearcytoplasmic or embryo-endosperm interactions may be involved. In the reciprocal F2, a relatively high frequency of maternal alleles was recovered for several loci, while the paternal allele was favored at others. The cDNA clone detecting the most extreme segregation, with no P. coccineus type detected among 165 P. vulgaris × P. coccineus F2progeny, showed high homology to histone H2A genes. The markers were mapped to nine linkage groups. Aggregation of markers with preferential maternal transmission was observed, which could be due to selection of individual chromosomes, although false linkage detection cannot be excluded. The results obtained with RFLPs may explain the skewed distribution of phenotypic traits following interspecific hybridization.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Crop Sci.Home page
L. C. Munoz, M. W. Blair, M. C. Duque, J. Tohme, and W. Roca
Introgression in Common Bean x Tepary Bean Interspecific Congruity-Backcross Lines as Measured by AFLP Markers
Crop Sci., March 1, 2004; 44(2): 637 - 645.
[Abstract] [Full Text] [PDF]


Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.