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The Journal of Heredity 1996:87(1):6-9
© 1996 The American Genetic Association 87:6-9

research-article

RFLP Mapping of Expressed Sequence Tags in the Chicken

C. A. Spike, N. Bumstead, L. B. Crittenden, and S. J. Lamont

From the Department of Animal Science, 201 Kildee Hall, Iowa State University Ames IA 50011-3150
the Institute for Animal Health, Compton Berkshire, U.K.
the Department of Microbiology, Giltner Hall, Michigan State University East Lansing, Michigan

Address correspondence to Dr. Lamont at the address above

Corresponding Editor: J. James Bitgood

Abstract

Chicken cDNA probes were used to map 10 loci by restriction fragment length polymorphism (RFLP) analysis in progeny of the Jungle Fowl x White Leghorn chicken backcross gene mapping reference population. The cDNA probes were from a subset of a T-cell library whose sequence tags showed significant homology to other avian or mammalian sequences in the EMBL database. Placement of loci generated by Compton cDNA clones on the East Lansing linkage map contributes to development of a consensus from the two linkage maps and will help provide a framework to evaluate the phenotypic effects of these genes.


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