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The Journal of Heredity 1996:87(3):252-258
© 1996 The American Genetic Association 87:252-258

other

Derivation and Characterization of a Somatic Cell Hybrid Containing the Portion of Mouse Chromosome 11 (MMU11) Homologous to Human Chromosome 17q

D. J. Morris-Rosendahl, G. Schriever-Schwemmer, and T. J. Robinson

From the Department of Zoology and Entomology, University of Pretoria Pretoria 0002, South Africa
Institut für Saugetlergenetik, GSF-Forschungszentrum für Umwelt und Gesundhelt Oberschlelssheim, Germany

Corresponding Editor. Christine Kozak

Abstract

To contribute to the physical gene map (mouse chromosome 11 (MMU11) and to extend the mapping resources available for this chromosome, we have produce mouse x rat somatic cell hybrids containing only bands B5 to E of MMU11. Characterization of the hybrids by polymerase chain reaction (PCR) amplification and Southern blot analyses of MMU11 markers revealed two hybrids, T16Ad14B and T16Ad19A, that had selectively retained the 311 translocation product containing distal MMU11 (bands B5-E). Cytogenetic analysis of the hybrid T16Ad14B by fluorescence in situ hybridization (FISH) and conventional G-banding confirmed the presence of the 311 translocation chromosome. Mapping of markers in both the T16Ad14B and T16Ad19A hybrids localized the T16Ad translocation breakpoint between the proximal markers Atplb2 and Acrb and the more distal markers Scya2 and Mpo. Loci for D11Mit5, Rpo2-1, Trp53, Glut4, Acrb, and Atplb2 could all be localized proximal to the T16Ad breakpoint in band B5, between bands B1 and B5 on MMU11.


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