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The Journal of Heredity 1996:87(5):399-403
© 1996 The American Genetic Association 87:399-403

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The Use of Transposable P-elements of Drosophila melanogaster for Introductory Genetics Laboratory Courses

P. Morcillo, R. Tuttle, and R. J. Maclntyre

Section of Genetics and Development Cornell University Ithaca, New York

Corresponding Editor: Stephen J. O'Brien

Abstract

Laboratories in introductory genetics courses often use Drosophila melanogaster as a tool for the students to understand how genes are transmitted and mapped. We demonstrate three advantages of using P-element containing inserts with the wildtype alleles of the genes to be mapped. First, inserts can be positioned at many places in the genome with relative ease; second, stocks can be chosen which have excellent viability; and third, inserts can be selected for precision in mapping with regard to marker genes. The results of a preliminary test with a beginning genetics laboratory are presented. The inserts exhibit excellent Mendelian ratios in F2 and backcross progeny and were reproducibly mapped by different student groups.


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