Skip Navigation

This Article
Right arrow FREE Full Text (PDF) Freely available
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (6)
Right arrowRequest Permissions
Google Scholar
Right arrow Articles by Biyashev, R. M.
Right arrow Articles by Saghai Maroof, M.A.
Right arrow Search for Related Content
PubMed
Right arrow Articles by Biyashev, R. M.
Right arrow Articles by Saghai Maroof, M.A.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

The Journal of Heredity 1997:88(1):21-26
© 1997 The American Genetic Association 88:21-26

research-article

Molecular Mapping, Chromosomal Assignment, and Genetic Diversity Analysis of Phytochrome Loci in Barley (Hordeum vulgare)

R. M. Biyashev, R. A. Ragab, P. J. Maughan, and M.A. Saghai Maroof

Department of Crop and Soil Environmental Sciences, Virginia Polytechnic Institute and State University Blacksburg, VA 24601

Abstract

Phytochromes belong to a family of photoreceptors that perceive, interpret, and translate light signals that effect plant growth and development The objectives of this study were to use a heterologous phytochrome cDNA probe from oat to (1) identify phytochrome loci in barley, (2) determine the chromosomal and genomic location of phytochrome gene(s) in barley, and (3) assess the extent of genetic variation at phytochrome loci in cultivated barley (Hordeum vulgare). Restriction fragment length polymorphism (RFLP) analysis detected four distinct phytochrome loci in barley, arbitrarily designated phy1, phy2, phy3, and phy4. Wheat-barley addition line analysis indicated that phyl, phy2, and phy3 are located on barley chromosomes 7, 4, and 5, respectively, while phy4 represents duplicate loci located on barley chromosomes 2 and 7. Linkage mapping confirmed our results using wheatbarley addition line analysis and located the phy1 and phy3 loci to the short arm of barley chromosome 7 and to the centromerlc region of barley chromosome 5, respectively. Two to three alleles were detected at each of the five phytochrome loci examined. Genetic diversity values for the phytochrome loci were near previous estimates for RFLPS. Division of the barley samples based on spike morphol ogy indicated that the level of diversity in two- and six-rowed barley is about equal, although significant differences in allelic frequencies were detected between the subgroups.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Crop Sci.Home page
J. A. Mammadov, J. C. Zwonitzer, R. M. Biyashev, C. A. Griffey, Y. Jin, B. J. Steffenson, and M. A. S. Maroof
Molecular Mapping of Leaf Rust Resistance Gene Rph5 in Barley
Crop Sci., January 1, 2003; 43(1): 388 - 393.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
M. Hanumappa, L. H. Pratt, M.-M. Cordonnier-Pratt, and G. F. Deitzer
A Photoperiod-Insensitive Barley Line Contains a Light-Labile Phytochrome B
Plant Physiology, March 1, 1999; 119(3): 1033 - 1040.
[Abstract] [Full Text]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.