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The Journal of Heredity 1997:88(6):489-494
© 1997 The American Genetic Association 88:489-494

research-article

Linkage Map for the Asian Tiger Mosquito [Aedes (Stegomyia) albopictus] Based on SSCP Analysis of RAPD Markers

J.-P. Mutebi, W. C. Black, IV, C. F. Bosio, W. P. Sweeney, Jr., and G. B. Craig, Jr.

Department of Biological Sciences, University of Notre Dame, Notre Dame Indiana
Department of Microbiology, Colorado State University Fort Collins, Colorado

Corresponding Editor: Martin Tracey

Abstract

A linkage map of the Asian tiger mosquito [Aedes (Stegomyla) albopictus (Skuse)] was constructed in an F1 intercross by monitoring the segregation of randomly amplified polymorphic DNA (RAPD) markers analyzed for single-strand conformation polymorphisms (SSCP). We hypothesized that SSCP analysis would reveal point mutations in RAPD fragments that would then segregate as codominant rather than dominant markers which are typically revealed through routine RAPD analysis. Markers were mapped to Individual chromosomes by testing for cosegregation with Sex (chromosome I) or a polymorphism at the a-GPD allozyme locus (chromosome II). All other markers that cosegregated were assigned to chromo some III. Six RAPD primers amplified 68 polymorphic markers that segregated in a Mendelian fashion and were mapped. Contrary to our hypothesis, no codominant SSCP polymorphisms were detected, but fractionation of RAPD products on polyacrylamide gels and detection through silver staining proved to be a sensitive tech nique that allowed us to identify more markers than the standard analysis of RAPD PCR products on agarose gels.


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