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The Journal of Heredity 1997:88(6):504-512
© 1997 The American Genetic Association 88:504-512

research-article

Identification of Rfp-Y (Mhc-like) Haplotypes in Chickens of Cornell Lines N and P

G. T. Pharr, R. L. Vallejo, and L. D. Bacon

College of Veterinary Medicine, Mississippi State University Mississippi State, Mississippi
U.S. Department of Agriculture, Agricultural Research Service, Avian Disease and Oncology Laboratory 3606 East Mount Hope Road, East Lansing, Michigan 48823

Corresponding Editor: Stephen E. Bloom

Abstract

Two strains of chickens selected for differential Marek's disease (MD) resistance or susceptibility were studied for the presence of the recently described Rfp-Y major histocompatibility complex (Mhc-like) haplotypes. MD resistant chickens from line N were fixed for the classical Mhc B21 haplotype, whereas MD susceptible line P chickens were fixed for the B19 haplotype. The Rfp-Y haplotypes were identified by restriction fragment polymorphism (RFP) analysis using enzymes and Mhc probes for B-G, B-LßII, and B-FIV. in addition an Rfp-LßIII clone was developed that differentiated Rfp-Lß from B-Lß genes. Three Rfp-Y haplotypes, defined for both class I and class II Mhc-like loci, were identified in line N (Rfp-Y5 and Rfp-Y7 and Rfp-Y9) and in line P (Rfp-Y5, Rfp-Y8, and Rfp-Y9 respectively. The Rfp-Y7 Rfp-Y8, and Rfp-Y9 haplotypes have not been described previously. The Rfp-Y5 haplotype was most frequent (0.70) in line N, but existed in low frequency (approximately 0.04) in line P; the Rfp-Y9 hapiotype was most frequent in line P (0.63), but was absent in line N. The Rfp-Y hapiotypes segregated in a Mendelian fashion in each line based on analysis of progeny from Rfp-Y heterozygous matings. The frequency of recombination between the Rfp-Y F and L loci was estimated to be less than 0.25%.


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[Abstract] [Full Text] [PDF]


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