The Journal of Heredity 1997:88(6):533-537
© 1997 The American Genetic Association 88:533-537
other |
Karyotypic Analysis of C-Banded Chromosomes of Diploid Alfalfa: Medicago sativa ssp. caerulea and ssp. falcata and Their Hybrid
USDA-ARS, Plant Sciences Institute, Soybean and Alfalfa Research Laboratory Beltsville, MD 20705-2350
Corresponding Editor Prem P. Jauhar
Abstract
Chromosomes of two diploid (2n = 2x = 16) subspecies of Medicago sativa ssp. caerulea and ssp. falcata and their hybrid were studied by C-banding. This study was undertaken to improve the C-banding technique for alfalfa chromosomes, develop a C-banded karyotype of the ssp. caerulea and ssp. falcata, and determine if the same C-banding technique could be used to identify parental chromosomes in hybrids. The chromosomes of ssp. falcata have only centromeric bands and thus individual chromosomes could not be identified. One accession of ssp. falcata displayed an interstitial band in the middle of the long arm on the satellite chromosome. However, chromosome-specific bands were observed in ssp. caerulea enabling the identification of each of the eight pairs of chromosomes and the development of a idiogram. All chromosomes had centromeric bands and a terminal band in the short arm except the satellite chromosome (chromosome 8). Interstitial bands were also observed in the short arms, with the exception of chromosome 7. Chromosomes 1, 2, 3, and 8 each had one prominent interstitial band in their long arm. The satellited chromosome is easy to identify because of the presence of the secondary constriction, two bands located on either side of the nucleolar organizer region, and a large terminal band on its long arm. The differences in banding patterns between these subspecies allowed the identification of parental chromosomes in hybrid cells.
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  X. Li and E. C. Brummer Inbreeding Depression for Fertility and Biomass in Advanced Generations of Inter- and Intrasubspecific Hybrids of Tetraploid Alfalfa Crop Sci., January 1, 2009; 49(1): 13 - 19. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. R. Bauchan, T. A. Campbell, and M. A. Hossain Comparative Chromosome Banding Studies of Nondormant Alfalfa Germplasm Crop Sci., November 1, 2003; 43(6): 2037 - 2042. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. R. Bauchan, T. A. Campbell, and M. A. Hossain Chromosomal Polymorphism as Detected by C-Banding Patterns in Chilean Alfalfa Germplasm Crop Sci., July 1, 2002; 42(4): 1291 - 1297. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. R. Bauchan and M. A. Hossain Distribution and Characterization of Heterochromatic DNA in the Tetraploid African Population Alfalfa Genome Crop Sci., November 1, 2001; 41(6): 1921 - 1926. [Abstract] [Full Text] [PDF]
|
|