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The Journal of Heredity 1998:89(1)
© 1998 The American Genetic Association 89:83-86

Brief communication. The chromosomes of Citrus and Poncirus species and hybrids: identification of characteristic chromosomes and physical mapping of rDNA loci using in situ hybridization and fluorochrome banding

ML Roose, T Schwarzacher, and JS Heslop-Harrison

In situ hybridization of 18S-5.8S-25S rDNA probes labeled with biotin or rhodamine and 5S rDNA probes labeled with digoxigenin was used to locate rDNA sites on root-tip metaphase chromosomes of Citrus sinensis L. (2n = 2x =18). Poncirus trifoliata L. Raf. (2n = 2x = 18), and Citrus sinensis X Poncirus trifoliata. (2n = 2x = 18). Counterstaining with the fluorochrome chomomycin A3 and DAPI uniquely identified many but not all chromosomes. C. sinensis had five 18S-25S rDNA sites, P. trifoliata had seven, and three different Citrus X Poncirus hybrids had five or six sites. Four 5S rDNA sites were detected, mostly linked to 18S-25S rDNA sites. Overall we observed high levels of chromosomal heterozygosity in all accessories examined.


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