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The Journal of Heredity 2000:91(3)
© 2000 The American Genetic Association 91:260-264

Brief communication. Karyotype analysis, banding, and fluorescent in situ hybridization in the scarab beetle Gymnopleurus sturmi McLeay (Coleoptera Scarabaeoidea: Scarabaeidae)

MS Colomba, R Vitturi*, and M Zunino

Dipartimento di Biologia Animale, Università di Palermo, Via Archirafi 18, 90123 Palermo, Italy *Corresponding author E-mail: zuvitcol@unipa.it

Conventional staining, differential banding, and in situ hybridization with both ribosomal and telomeric probes to mitotic chromosomes of Gymnopleurus sturmi (Scarabaeoidea: Scarabaeidae) are described. The karyotype is distinguished by a pericentric inversion polymorphism in chromosome 3, which is either acrocentric or subtelocentric. Silver staining (Ag-NOR) and chromomycin A3 (CMA3), failed to study the detection of nucleolar organizer regions (NORs), due to the extensive silver and CMA3 stainability of all GC-rich heterochromatin. Fluorescent in situ hybridization (FISH) using a Paracentrotus lividus (Echinodermata) rDNA probe mapped the ribosomal RNA genes (rDNA). FISH with the all-human telomeric sequences (TTAGGG)n revealed a lack of homology between the telomeric probe and the telomeres of G. sturmi. This suggests that the telomeric hexanucleotide (TTAGGG)n is not so conserved within eukaryotes as it has been hypothesized.


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