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The Journal of Heredity 2000:91(5)
© 2000 The American Genetic Association 91:392-396

Soybean genetic map of RAPD markers assigned to an existing scaffold RFLP map

AR Ferreira1,*, KR Foutz2, and P Keim3

1Rigesa Ltda, Dep. Florestal, Caixa Postal 31, Canoinhas, SC 89460-000, Brazil 2Westvaco, Forest Science and Technology, Summerville, South Carolina, USA 3Northern Arizona University, Department of Biological Sciences, Flagstaff, Arizona, USA *Corresponding author E-mail: arnaldo.ferreira@rigesa.com.br

A 356-marker linkage map of Glycine max (L.) Merr. (2n = 20) was established by anchoring 106 RAPD markers to an existing RFLP map built with a large recombinant inbred line population (330 RILs). This map comprises 24 major and 11 minor linkage groups for this genome which is estimated to be approximately 3275 cM. The RAPD markers show similar distribution throughout the genome and identified similar levels of polymorphism as the RFLP markers used in the framework. By using a subset population to anchor the RAPD markers, it was possible to enhance the throughput of selecting and adding reliable marker loci to the existing map. The procedures to generate a dependable genetic linkage map are also described in this report.


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