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The Journal of Heredity 2000:91(5)
© 2000 The American Genetic Association 91:412-415

Brief communication. Characterization and chromosomal distribution of a novel satellite DNA sequence of Japanese quail (Coturnix coturnix japonica

K Tanaka1,*, T Suzuki1, T Nojiri1, T Yamagata1, T Namikawa1, and Y Matsuda1,2

1Laboratory of Animal Genetics, Graduate School of Bioagricultural Sciences, Nagoya University, Chikusa, Nagoya 464-8601, Japan 2Chromosome Research Unit, Faculty of Science, and Laboratory of Cytogenetics, Division of Bioscience, Graduate School of Environmental Earth Science, Hokkaido University, Sapporo, Japan *Corresponding author E-mail: kztana@agr.nagoya-u.ac.jp

A novel satellite DNA sequence of Japanese quail (Coturnix coturnix japonica was isolated from genomic DNA digested with restriction endonuclease, Bg/II. Sequence analysis of three different-size clones revealed the presence of a tandem array of a GC-rich 41 bp repeated element. This sequence was localized by fluorescence in situ hybridization (FISH) primarily to microchromosomes of Japanese quail (2n = 78); approximately 50 of the 66 microchromosomes showed positive signals, although hybridization signals were also detected on chromosomes 4 and W. This satellite DNA did not cross-hybridize with genomic DNA of chicken (Gallus gallus) and Chinese painted quail (Excalfactoria chinensis) under moderately stringent conditions, suggesting that this class of repetitive DNA sequences was species specific and fairly divergent in Galliformes species.


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