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The Journal of Heredity 2001:92(1)
© 2001 The American Genetic Association 92:81-83

Brief Communication

Production of Wheat Doubled Haploids by Pollination With Job's Tears (Coix lachryma-jobi L.)

K. Mochida, and H. Tsujimoto

From the Graduate School of Integrated Science and Kihara Institute for Biological Research, Yokohama City University, 641-12 Maioka-cho, Totsuka-ku, Yokohama 244-0813, Japan.

Wheat (Triticum aestivum L.) haploids were produced by crossing with Job's tears (Coix lachryma-jobi L.) as the pollen parent. Pollination was followed by 2,4-D treatment, detached tiller culture, and embryo culture, as described for maize pollination. The frequency of embryo formation was similar to that obtained by crossing wheat with maize pollen. Job's tears is a perennial plant which forms several stalks and its pollen can be collected throughout the year when the plant is maintained in a controlled environment. Our results indicate that Job's tears can be used as the pollen parent for wheat crosses for haploid production without requiring synchronization of flowering dates.


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P. P. Jauhar, S. S. Xu, and P. S. Baenziger
Haploidy in Cultivated Wheats: Induction and Utility in Basic and Applied Research
Crop Sci., May 11, 2009; 49(3): 737 - 755.
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