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The Journal of Heredity 2001:92(5)
© 2001 The American Genetic Association 92:392-397

Isolation of a Sex-Linked DNA Sequence in Cranes

W. Duan, and P. A. Fuerst

From the Department of Molecular Genetics, Ohio State University, 484 W 12th Ave. Columbus, OH 43210.

Address correspondence to Paul A. Fuerst at the address above or e-mail: fuerst.1{at}osu.edu.

A female-specific DNA fragment (CSL-W; crane sex-linked DNA on W chromosome) was cloned from female whooping cranes (Grus americana). From the nucleotide sequence of CSL-W, a set of polymerase chain reaction (PCR) primers was identified which amplify a 227–230 bp female-specific fragment from all existing crane species and some other noncrane species. A duplicated versions of the DNA segment, which is found to have a larger size (231–235 bp) than CSL-W in both sexes, was also identified, and was designated CSL-NW (crane sex-linked DNA on non-W chromosome). The nucleotide similarity between the sequences of CSL-W and CSL-NW from whooping cranes was 86.3%. The CSL primers do not amplify any sequence from mammalian DNA, limiting the potential for contamination from human sources. Using the CSL primers in combination with a quick DNA extraction method allows the noninvasive identification of crane gender in less than 10 h. A test of the methodology was carried out on fully developed body feathers from 18 captive cranes and resulted in 100% successful identification.


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