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The Journal of Heredity 2002:93(1)
© 2002 The American Genetic Association 93:63-66

Brief Communication

Heterologous Amplification of Microsatellite Markers From Colubroid Snakes in European Natricines (Serpentes: Natricinae)

A. Hille, I. A. W. Janssen, S. B. J. Menken, M. Schlegel, and R. S. Thorpe

From the Zoologische Staatssammlung München, Muenchhausenstrasse 21, D-81247 Munich, Germany (Hille), Faculty of Biosciences, Zoology Department, University of Leipzig, Leipzig, Germany (Hille, Janssen, and Schlegel), Institute for Biodiversity and Ecosystem Dynamics, University of Amsterdam, Amsterdam, The Netherlands (Janssen and Menken), and School of Biological Sciences, University of Wales, Bangor, Gwynedd, United Kingdom (Thorpe).

Address correspondence to Axel Hille at the address above or e-mail: axel.hille{at}zsm.mwn.de.

Eighteen microsatellite loci developed for a range of snake species (New World natricines, elapids, crotalids) were tested against European natricines (Natrix natrix, N. maura, and N. tessellata) in cross-species amplification experiments. Five loci were polymorphic (average expected heterozygosity 0.749 for a population of N. natrix in Amsterdam, mean sample size 47.8) and three loci were monomorphic. The remainder could not be consistently scored or failed to amplify. Further tests on single individuals of a diverse set of eight species of colubroid snakes showed that 15 of the 18 loci could be cross-amplified in at least one of these species. We conclude that our results show promise for the utilization of these markers for experimental assessments of genetic variation in the phylogenetically closely related group of European natricine snakes with emphasis on N. natrix. The full suite of microsatellite markers now available for snakes may show additional potential for subsequent investigation across a broader range of colubroid snakes.


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