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The Journal of Heredity 2002:93(4)
© 2002 The American Genetic Association 93:300-302

Brief Communication

RAPD Analysis Reveals Genetic Variability Among Sexual and Apomictic Paspalum dilatatum Poiret Biotypes

A. M. Casa, S. E. Mitchell, C. R. Lopes, and J. F. M. Valls

From the Universidade Estadual Paulista (UNESP), Department of Genetics, Rubião Junior, Botucatu, SP, 18618-000 Brazil (Casa and Lopes); Cornell University Institute for Genomic Diversity, 151 Biotechnology Building, Ithaca, NY 14853 (Mitchell); and Embrapa Recursos Genéticos e Biotecnologia, Parque Estação Biológica, Brasília, DF, 70770-900 Brazil (Valls).

Address correspondence to Alexandra M. Casa at the address above, or e-mail: amc56{at}cornell.edu.

Paspalum dilatatum is a valuable forage grass in the subtropics. This species consists of several sexual (tetraploid) and apomict (penta- and hexaploid) biotypes. It has been proposed that the presence of a genome of unknown origin, the X genome, is responsible for apomixis in penta- and hexaploid biotypes. Here we evaluated the utility of random amplified polymorphic DNA (RAPD) markers for discriminating sexual and apomictic P. dilatatum biotypes. DNA samples from nine accessions, including P. intermedium, P. juergensii, and P. dilatatum (ssp. flavescens, and the common and Uruguayan biotypes) were analyzed with 86 RAPD primers. Three hundred sixty-two fragments were scored and genetic similarity estimates revealed that the penta- and hexaploid biotypes were highly similar (SD >= 0.913). Forty RAPDs were unique to the penta- and hexaploid biotypes. Overall RAPD markers were useful for assessing genetic variation among closely related P. dilatatum genotypes as well as generating putative X genome markers.


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