Skip Navigation

This Article
Right arrow Full Text Freely available
Right arrow FREE Full Text (PDF) Freely available
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (10)
Right arrowRequest Permissions
Google Scholar
Right arrow Articles by Casa, A. M.
Right arrow Articles by Valls, J. F. M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Casa, A. M.
Right arrow Articles by Valls, J. F. M.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?


The Journal of Heredity 2002:93(4)
© 2002 The American Genetic Association 93:300-302

Brief Communication

RAPD Analysis Reveals Genetic Variability Among Sexual and Apomictic Paspalum dilatatum Poiret Biotypes

A. M. Casa, S. E. Mitchell, C. R. Lopes, and J. F. M. Valls

From the Universidade Estadual Paulista (UNESP), Department of Genetics, Rubião Junior, Botucatu, SP, 18618-000 Brazil (Casa and Lopes); Cornell University Institute for Genomic Diversity, 151 Biotechnology Building, Ithaca, NY 14853 (Mitchell); and Embrapa Recursos Genéticos e Biotecnologia, Parque Estação Biológica, Brasília, DF, 70770-900 Brazil (Valls).

Address correspondence to Alexandra M. Casa at the address above, or e-mail: amc56{at}cornell.edu.

Paspalum dilatatum is a valuable forage grass in the subtropics. This species consists of several sexual (tetraploid) and apomict (penta- and hexaploid) biotypes. It has been proposed that the presence of a genome of unknown origin, the X genome, is responsible for apomixis in penta- and hexaploid biotypes. Here we evaluated the utility of random amplified polymorphic DNA (RAPD) markers for discriminating sexual and apomictic P. dilatatum biotypes. DNA samples from nine accessions, including P. intermedium, P. juergensii, and P. dilatatum (ssp. flavescens, and the common and Uruguayan biotypes) were analyzed with 86 RAPD primers. Three hundred sixty-two fragments were scored and genetic similarity estimates revealed that the penta- and hexaploid biotypes were highly similar (SD >= 0.913). Forty RAPDs were unique to the penta- and hexaploid biotypes. Overall RAPD markers were useful for assessing genetic variation among closely related P. dilatatum genotypes as well as generating putative X genome markers.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?




Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.