Journal of Heredity 2003:94(1)
© 2003 The American Genetic Association 94:39-48
Genetic Structure of Susceptibility Traits for Hip Dysplasia and Microsatellite Informativeness of an Outcrossed Canine Pedigree
From the Department of Clinical Sciences (Todhunter, Bliss, and Gilbert) and the James A. Baker Institute for Animal Health (Acland, Lust, and Williams), College of Veterinary Medicine, Cornell University, Ithaca, NY, and the Department of Statistics, University of Florida, Gainesville, FL (Wu and Casella).
Address correspondence to Rory Todhunter, Department of Clinical Sciences, Box 32, College of Veterinary Medicine, Cornell University, Ithaca, NY 14850, or e-mail: rjt2{at}cornell.edu.
An outcrossed canine pedigree was developed for quantitative trait locus (QTL) mapping of hip dysplasia by breeding dysplastic Labrador retrievers to trait-free greyhounds. Measured susceptibility traits included age at onset of femoral capital chondroepiphyseal ossification (OSS), maximum hip distraction (laxity) index (DI), and the dorsolateral subluxation (DLS) score. The pedigrees consisted of 147 dogs representing four generations. For 59 dogs genotyped with 65 microsatellites, the median heterozygosity and polymorphic information content (PIC) values of the F1 generation were 0.82 and 0.68, respectively. Seventy-seven percent of microsatellites had a PIC greater than 0.59 in the F1s. Ninety-six percent of alleles showed Mendelian inheritance. Based on marker informativeness, approximately 350 randomly selected markers would be required for genome-wide screening to obtain an average interval between informative markers of 10 cM. Heritability was estimated as 0.43, 0.5, and 0.61 for OSS, DI, and the DLS score, respectively. Biometric estimates of the mean (± variance) effective number of segregating QTLs was 1.2 (± 0.05), 0.8 (± 0.02), and 1.0 (± 0.03) for OSS, DI, and the DLS score, respectively. The distributions of simulated backcross trait data suggested that the loci controlling these traits acted additively and that the DI may be controlled by a major locus. When combined with previous power and quantitative genetic analyses, these estimates indicate that this pedigree is informative for QTL mapping of hip dysplasia traits.
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