Journal of Heredity 2003:94(2)
© 2003 The American Genetic Association 94:171-174
Brief Communication |
Nectomys squamipes Microsatellites and Homologous Loci in Sigmodontine Rodents
From the Genetics Department, Universidade Federal do Rio de Janeiro, 68011 Cidade UniversitÄÄria, 21944-970 Rio de Janeiro, RJ, Brazil (Maroja, Almeida, and Seuánez); the Ecology Department, Universidade Federal do Rio de Janeiro, Caixa Postal 68020, 21491-590 Rio de Janeiro, RJ, Brazil (Cerqueira); and the Genetics Division, Instituto Nacional de Cancer, Praça da Cruz Vermelha 23, Sexto Andar, 20230-130 Rio de Janeiro, RJ, Brazil (Seuánez and Moreira). Work funded by CNPq-PRONEX, PROBIO/MMA, FIOCRUZ, FUJB, INCa/FAF, and CAPES graduate grants (Brazil) (L.S.M. and F.C.A.).
Address correspondence to H. N. Seuánez at the above address, or e-mail: genetics{at}inca.gov.br.
Three monomorphic and four highly polymorphic microsatellites of Nectomys squamipes were isolated and characterised in a sample of 141 specimens from eight different Brazilian localities. These seven microsatellites and four others previously described in this species were tested in seven other nonfocus sigmodontine species. At least three loci were successfully amplified in every species, but none was amplified in all species. All sequenced products in nonfocus species showed (GT)n motifs as in N. squamipes. Several loci were amplified in Nectomys rattus and Oligoryzomys nigripes, while absence of PCR products was observed more frequently in Oxymycterus dasythricus and Akodon cursor. Two of three monomorphic loci in N. squamipes were polymorphic in other species.