Skip Navigation

This Article
Right arrow Full Text Freely available
Right arrow FREE Full Text (PDF) Freely available
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (11)
Right arrowRequest Permissions
Google Scholar
Right arrow Articles by Zhong, D.
Right arrow Articles by Yan, G.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Zhong, D.
Right arrow Articles by Yan, G.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Journal of Heredity 2004:95(1)
© 2004 The American Genetic Association 95:53-61

AFLP-Based Genetic Linkage Map for the Red Flour Beetle (Tribolium castaneum)

D. Zhong, A. Pai, and G. Yan

From the Department of Biological Sciences, State University of New York at Buffalo, Buffalo, NY 14260. We thank P. Zwiers, M. Karsay-Klein, and C. Costa for technical assistance. This research was supported by a grant from the National Science Foundation (DEB 0076106). Two anonymous reviewers provided constructive suggestions.

Address correspondence to Guiyun Yan at the address above, or e-mail: gyan{at}buffalo.edu.

The red flour beetle (Tribolium castaneum) is a major pest of stored grain and grain products and a popular model species for a variety of ecological, evolutionary, and developmental biology studies. Development of a linkage map based on reproducible and highly polymorphic molecular markers would greatly facilitate research in these disciplines. We have developed a genetic linkage map using 269 amplified fragment length polymorphism (AFLP) markers. Ten previously known random amplified polymorphic DNA (RAPD) markers were used as anchor markers for linkage group assignment. The linkage map was constructed through genotyping two independent F2 segregating populations with 48 AFLP primer combinations. Each primer combination generated an average of 4.6 AFLP markers eligible for linkage mapping. The length of the integrated map is 573 cM, giving an average marker resolution of 2.0 cM and an average physical distance per genetic distance of 350 kb/cM. A cluster of loci on linkage group 3 exhibited significant segregation distortion. We have also identified six X-linked and two Y-linked markers. Five mapped AFLP fragments were sequenced and converted to sequence-tagged site (STS) markers.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 GeneticsHome page
D. Zhong, D. M. Menge, E. A. Temu, H. Chen, and G. Yan
Amplified Fragment Length Polymorphism Mapping of Quantitative Trait Loci for Malaria Parasite Susceptibility in the Yellow Fever Mosquito Aedes aegypti
Genetics, July 1, 2006; 173(3): 1337 - 1345.
[Abstract] [Full Text] [PDF]

Home page
 GeneticsHome page
D. Zhong, E. A. Temu, T. Guda, L. Gouagna, D. Menge, A. Pai, J. Githure, J. C. Beier, and G. Yan
Dynamics of Gene Introgression in the African Malaria Vector Anopheles gambiae
Genetics, April 1, 2006; 172(4): 2359 - 2365.
[Abstract] [Full Text] [PDF]

Home page
 GeneticsHome page
M. D. Lorenzen, Z. Doyungan, J. Savard, K. Snow, L. R. Crumly, T. D. Shippy, J. J. Stuart, S. J. Brown, and R. W. Beeman
Genetic Linkage Maps of the Red Flour Beetle, Tribolium castaneum, Based on Bacterial Artificial Chromosomes and Expressed Sequence Tags
Genetics, June 1, 2005; 170(2): 741 - 747.
[Abstract] [Full Text] [PDF]

Home page
 GeneticsHome page
D. Zhong, A. Pai, and G. Yan
Costly Resistance to Parasitism: Evidence From Simultaneous Quantitative Trait Loci Mapping for Resistance and Fitness in Tribolium castaneum
Genetics, April 1, 2005; 169(4): 2127 - 2135.
[Abstract] [Full Text] [PDF]


Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.