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Journal of Heredity 2004:95(1)
© 2004 The American Genetic Association 95:62-69

A Minisatellite with Fold-Back Structure is Included in the 5'-Flanking Region of the Adh Gene of Scaptodrosophila lebanonensis

D. J. Orengo, M. Papaceit, and E. Juan

From the Departament de Genètica, Facultat de Biologia, Universitat de Barcelona, Avda. Diagonal 645, 08028 Barcelona, Spain. This work was supported by DGICYT grant PB96-0172 and BOS2000-0770 (to E.J.) and by CIRIT grant 1999SGR-25 (to M. Aguadé). We thank Serveis Cientificotècnics, Universitat de Barcelona for automated sequencing facilities.

Address correspondence to D. J. Orengo at the address above, or e-mail: dorcas{at}porthos.bio.ub.es.

A tandem repetitive sequence with a repeat unit of 12 bp has been found 1.3 kb upstream of the Adh gene of Scaptodrosophila lebanonensis. This repetitive sequence extends over 4.3 kb and consists of two inverted arrays (a fold-back segment). The repeated unit with a consensus sequence GAATACAGAATA is highly conserved and the nucleotide substitutions are not distributed randomly among the 12 bp. In situ hybridization in S. lebanonensis polytene chromosomes revealed two signals, one at the 60A section, the Adh locus, and a second site in the same chromosome at the 60C section close to the telomere. This same pattern of hybridization is obtained in all the analyzed strains including the subspecies S. lebanonensis casteeli. The minisatellite sequence accounts for about 0.03–0.04% of the S. lebanonensis genome and showed intraspecific variability in tandem repeat numbers. Possible functions of this sequence are discussed.


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M. Papaceit, D. Orengo, and E. Juan
Sequences Upstream of the Homologous cis-elements of the Adh Adult Enhancer of Drosophila Are Required for Maximal Levels of Adh Gene Transcription in Adults of Scaptodrosophila lebanonensis
Genetics, May 1, 2004; 167(1): 289 - 299.
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