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Journal of Heredity 2004:95(2):158-164
© 2004 The American Genetic Association

Brief Communication

Isolation of Y Chromosome-specific Microsatellites in the Horse and Cross-species Amplification in the Genus Equus

B. Wallner, F. Piumi, G. Brem, M. Müller, and R. Achmann

From the Institut für Tierzucht und Genetik, Veterinärmedizinische Universität Wien Veterinärplatz 1, A-1210 Wien, Austria (Wallner, Brem, and Müller); INRA, Centre de Recherches de Jouy, Departement de Genetique Animale, Jouy-en-Josas, France (Piumi); and Ludwig Boltzmann-Institut für immuno-, zyto- und molekulargenetische Forschung, Veterinärplatz 1, A-1210 Wien, Austria (Brem and Achmann). We thank W. Zimmermann, C. Waltzer, H. Korb, E. Dvorak, H. Siegismund, the Zoological Society of San Diego, and the Zoological Garden Munich for samples. We are grateful to M. Wigler for a detailed RDA protocol. Thanks to G. Muir for comments on the manuscript. This study was financially supported by the Hochschuljubiläumsstiftung der Stadt Wien (H-234/99) and Agrobiogen GmbH.

Address correspondence to Roland Achmann, genteQ GmbH, Falkenried 88, D-20251 Hamburg, Germany, or e-mail: achmann{at}genteQ.de.

Y chromosome polymorphisms such as microsatellites or single nucleotide polymorphisms represent a paternal counterpart to mitochondrial DNA (mtDNA) for evolutionary and phylogeographic studies. The use of Y chromosome haplotyping in natural populations of species other than humans is still hindered by the lack of sequence information necessary for polymorphism screening. Here we used representational difference analysis (RDA) followed by a screen of a bacterial artificial chromosome (BAC) library for repetitive sequences to obtain polymorphic Y-chromosomal markers. The procedure was performed for the domestic horse (Equus caballus) and we report the first six Y-chromosomal microsatellite markers for this species. Three markers were also useful for haplotyping taxa of the zebra/ass lineage. Y-chromosomal microsatellite markers show a single haplotype in the domestic horse, whereas notable variation has been observed in the other members of the genus Equus.


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