© 2004 The American Genetic Association
Brief Communication |
Genotyping of Mature Trees of Entandrophragma cylindricum with Microsatellites
From Centre de Coopération Internationale en Recherche Agronomique pour le Développement-Forêt, Campus International de Baillarguet, TA 10/B, F34398, Montpellier Cedex 5, France (Garcia and Chevallier), and Centre de Coopération Internationale en Recherche Agronomique pour le Développement, UMR, 1096/PIA, TA 40/03, F34398, Montpellier Cedex 5, France (Noyer and Risterucci). M.-H. Chevallier is currently at Centre d'Ecologie Fonctionelle et Evolutive, 1919 route de Mende, F34293, Montpellier Cedex 5.
Address correspondence to M.-H. Chevallier at the above address, or e-mail: chevallier{at}cefe.cnrs-mop.fr.
We have characterized 10 microsatellite loci for the tropical tree Entandrophragma cylindricum (Sprague) Sprague (sapelli) in order to genotype individuals in forest stands for estimation of the genetic diversity of the species. We used the technique of building a (GA)n microsatellite–enriched library by capture with streptavidin-coated magnetic beads. We assessed the polymorphism of seven microsatellites in 186 mature trees in a selectively logged stand (Dimako) and an unlogged stand (Ndama), both in Cameroon. All the loci were polymorphic, and the number of alleles was high, ranging from eight to 36, with a mean of 22.1. Both stands showed the same high level of genetic diversity (mean HE = 0.85) and a low genetic differentiation (FST = 0.007), indicating that genetic diversity was within rather than among populations. Five and three out seven loci in Dimako and Ndama, respectively, showed a deficit of heterozygotes. The seven loci enabled more than 97% of the mature trees in each stand to be identified. It was concluded that these markers can be efficiently used for gene flow studies.