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Journal of Heredity Advance Access originally published online on December 14, 2004
Journal of Heredity 2005 96(1):80-82; doi:10.1093/jhered/esi015
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© 2005 The American Genetic Association

Computer Note

Expeditor: A Pipeline for Designing Primers Using Human Gene Structure and Livestock Animal EST Information

Z.-L. Hu, K. Glenn, A. M. Ramos, C. J. Otieno, J. M. Reecy, and M. F. Rothschild

From the Department of Animal Science, 2255 Kildee Hall, Center for Integrated Animal Genomics, Iowa State University, Ames, IA 50011

Address correspondence to Zhi-Liang Hu and Max F. Rothschild at the address above, or e-mail: zhu{at}iastate.edu or mfrothsc{at}iastate.edu, respectively.

We have developed software, called Expeditor, that can be used to combine known gene structure information from human and coding sequence information from farm animal species for a streamlined primer design in target farm animal species. This software has many utilities, which include PCR-based SNP discovery for identification of genes/markers associated with economically important traits in farm animals, comparative mapping analysis, and evolution studies. The use of this software helps minimize tedious manual operations and reduces the chance of errors by more conventional approaches.


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