Skip Navigation


Journal of Heredity Advance Access originally published online on December 15, 2004
Journal of Heredity 2005 96(2):161-166; doi:10.1093/jhered/esi023
This Article
Right arrow Full Text Freely available
Right arrow FREE Full Text (PDF) Freely available
Right arrow All Versions of this Article:
96/2/161    most recent
esi023v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (7)
Right arrowRequest Permissions
Google Scholar
Right arrow Articles by Kirst, M.
Right arrow Articles by Grattapaglia, D.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Kirst, M.
Right arrow Articles by Grattapaglia, D.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

© The American Genetic Association. 2004. All rights reserved. For Permissions, please email: journals.permissions@oupjournals.org

Brief Communication

Power of Microsatellite Markers for Fingerprinting and Parentage Analysis in Eucalyptus grandis Breeding Populations

M. Kirst, C. M. Cordeiro, G. D. S. P. Rezende, and D. Grattapaglia

From the Plant Genetics Laboratory – EMBRAPA Recursos Genéticos e Biotecnologia, Parque Estação Biológica, Brasília 70770-970 DF, Brazil (Kirst, Cordeiro, and Grattapaglia); Research and Technology Center – Aracruz Celulose S.A. C.P. 331011 Aracruz 29197-000 ES, Brazil (Rezende); and Genomic Sciences Laboratory, Universidade Catolica de Brasília – SGAN 916 modulo B, Brasília 70790-160 DF (Grattapaglia)

Address correspondence to Dario Grattapaglia, Laboratório de Genética de Plantas – EMBRAPA Recursos Genéticos e Biotecnologia, Parque Estação Biológica, Brasília 70770-970 DF, Brazil, or e-mail: dario{at}cenargen.embrapa.br.

We report the genetic analysis of 192 unrelated individuals of an elite breeding population of Eucalyptus grandis (Hill ex Maiden) with a selected set of six highly polymorphic microsatellite markers developed for species of the genus Eucalyptus. A full characterization of this set of six loci was carried out generating allele frequency distributions that were used to estimate parameters of genetic information content of these loci, including expected heterozygosity, polymorphism information content (PIC), power of exclusion, and probability of identity. The number of detected alleles per locus ranged from 6 to 33, with an average of 19.8 ± 9.2. The average expected heterozygosity was 0.86 ± 0.11 and the average PIC was 0.83 ± 0.16. Using only three loci, it was possible to discriminate all 192 individuals. The overall probability of identity considering all six EMBRA microsatellite markers combined was lower than 1 in 2 billion. An analysis of the sample size necessary to estimate expected heterozygosity with minimum variance indicated that at least 64 individuals have to be genotyped to characterize this parameter with adequate accuracy for most microsatellites in Eucalyptus. The high degree of multiallelism and the clear and simple codominant Mendelian inheritance of the set of microsatellites used provide an extremely powerful system for the unique identification of Eucalyptus individuals for fingerprinting purposes and parentage testing.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?




Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.