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Journal of Heredity Advance Access originally published online on July 1, 2005
Journal of Heredity 2005 96(5):576-581; doi:10.1093/jhered/esi076
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© The American Genetic Association. 2005. All rights reserved. For Permissions, please email: journals.permissions@oupjournals.org.

Brief Communication

Inferences on the Role of Insertion in a Mutation Accumulation Experiment with Drosophila melanogaster Using RAPDs

C. Salgado, B. Nieto, M. A. Toro, C. López-Fanjul, and A. García-Dorado

From the Departamento de Producción Animal, Facultad de Veterinaria, Universidad Complutense, 28040 Madrid (Salgado and Nieto); and Departamento de Mejora Genética Animal, INIA, 28040 Madrid (Toro); and Departamento de Genética, Facultad de Biología, Universidad Complutense, 28040 Madrid (López-Fanjul and García-Dorado)

Address correspondence to A. García-Dorado at the address above, or e-mail: augardo{at}bio.ucm.es.

The genetic variability for RAPDs band pattern was studied in a set of 157 mutation accumulation (MA) lines of Drosophila melanogaster. These MA lines were derived from the same isogenic base population and subsequently maintained by full-sib mating during 132 generations. The ancestral pattern of the original isogenic base can be unambiguously established as the consensus pattern of the MA lines and, because these lines are expected to be homozygous, dominance for band pattern is not a concern. Only repeatable changes in band pattern were considered. The number of ancestral bands detected implies that nine-nucleotide targets are enough for repeatable PCR amplification. Compared with the ancestral pattern, one MA line lost one band and two MA lines gained a new one. These results can be accounted for by the insertion of transposable elements occurring at a rate 0.07 < i < 0.21 per whole haploid genome and generation. This range is typical for Drosophila and consistent with the previously observed mobility for the roo family, supporting the generality of previous estimates of spontaneous mutation rates for morphological and fitness traits based on these MA lines. The sequence of one of the new bands suggests that the Idefix family is also active in the lines.


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M. Papaceit, V. Avila, M. Aguade, and A. Garcia-Dorado
The Dynamics of the roo Transposable Element In Mutation-Accumulation Lines and Segregating Populations of Drosophila melanogaster
Genetics, September 1, 2007; 177(1): 511 - 522.
[Abstract] [Full Text] [PDF]


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